Isolation of HSCs or LSCs by cell surface markers is better suited for subsequent functional studies such as in vivo transplantation since the Hoechst dye is toxic to the cells and therefore might impair their physiological functions.
hMSCs were isolated and identified by cell surface markers, then, cultured in Minimum Essential Medium Alpha Medium (α-MEM) supplemented with 10% FBS, 2 mM L-Glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin, and incubated in a humidified atmosphere of 5% CO2 at 37°C.
Having observed that SALL4 was enriched in HS/PCs that were identified by cell surface markers, we planned to evaluate the expression of this stem cell factor in SP and non-SP cells in various blood cancer cell lines (HL-60, NB4, KG1a and RPMI8226 cells), as well as investigate the role of SALL4 in drug resistance and cancer stem cells as identified by SP studies.
However, a maturation status of a macrophage might be more complex than just defined by cell surface markers or morphology.
Here we used a FACS-based technique that allowed us to identify producer cells by cell surface markers and cytokine production simultaneously (13).
This suggests two populations of cells may have the same "apparent" state as measured by cell surface markers while differing in other state space dimensions, ultimately leading them to disparate cell fates.
This has been most convincingly demonstrated by examining the ability of subpopulations of tumor cells identified by cell surface markers to form tumors when transplanted into immunosuppressed NOD/SCID mice.
Multiple studies have examined stem/progenitor activity in populations defined by cell surface markers [ 11, 16, 20, 22, 30, 31], but have reported conflicting results about whether luminal or basal lineages were enriched for progenitor activity.
