Sentence examples for by cell surface biotinylation from inspiring English sources
However, despite several attempts, EWI-2wint expression in cellular clones could not be detected by cell surface biotinylation and immunoprecipitation or by western blotting experiments (data not shown), suggesting that this protein was poorly expressed in these cells.
We compared the amount of EphA4 expressed on the cell surface; this was estimated by cell surface biotinylation, in NIH3T3 cells transformed with EphA4-HA, with or without the exogenous expression of Meltrin β (Fig. 5A).
Indeed, 200 nM RAP did not prevent constitutive internalization of uPAR in HEK293-uPAR cells as demonstrated by cell surface biotinylation and immunoblotting of the cell extracts, as well as by immunofluorescence analysis.
Furthermore, in A549 cells expressing a rat Na,K-ATPase α1-subunit where the Ser-18 residue was mutated to alanine (S18A), the high CO2-induced Na,K-ATPase endocytosis was prevented as assessed by cell surface biotinylation and live cell imaging with epi-fluorescent microscopy (Figure 6B and 6C).
These membrane fractionation studies were reinforced by cell surface biotinylation assays (Fig. 7b).
The cellular localization of the expressed transporters was investigated by cell surface biotinylation.
Precursor cleavage was demonstrated by cell-surface biotinylation and Western blotting.
The presence of an abundant surface exposed pool of Syb2 selectively in TKO MEFs was confirmed by the surface immunofluorescence for the HA epitope and by a cell surface biotinylation assay.
Since only MT1-MMP expressed on the cell surface mediates degradation of the extracellular matrix [ 32], we next evaluated its surface expression by both cell surface biotinylation and flow cytometry analysis, as described in Materials and Methods.
By coimmunoprecipitation, ECM1 was found to interact physically with EGFR, and, by immunostaining and cell surface biotinylation, ECM1 was detected at the cell surface.
Furthermore, by performing a cell surface biotinylation assay we ensured that at least a detectable fraction of all the TRKB mutants properly localized to the cell membrane [36] (Figure 1D).
