We were able to obtain information on protein localization in the volume of cells by correlating the oligomerization of GFP-tagged MTM1 with immunogold labeling observed by transmission-EM or with cell surface analysis done by scanning-EM.
The isolation and characterization of human UCB-MSCs were performed by cell surface marker analysis and multilineage differentiation in our previous report.
The cell surface properties of demulsifying bacteria cells can be controlled by cell surface substances.
CD34+ cells were initially enriched from human fetal liver (ABR Inc., Alameda, CA, USA) or human cord blood (NDRI, Philadelphia, PA, USA) using the EasySep Human CD34 Positive Selection Kit (Stem Cell Technologies, Vancouver, Canada) for cell surface marker analysis by flow cytometry.
After validation of the reporter system, we set out to characterize the cellular identity of PI-MECs by FACS analysis by using cell surface markers for the various epithelial populations of the mammary gland.
Finally, we chose to examine the expression of CCR5 and CXCR4, the 2 major HIV-1 co-receptors, on the cell surface by FACS analysis as the first part of our ongoing efforts to verify our array data and analysis.
To confirm P31-43 induced alterations of the recycling vesicular compartment, we investigated the levels of recycling marker transferrin receptor on the cell surface by FACS analysis before and after overnight treatment with P31-43 or P57-68 P57-68
Confirmation that the O1-Ag was present on the cell surface by FACS analysis demonstrated that the O1-Ag saccharides alone on the cell surface were unable to protect against lysis by complement, further validating findings that the capsule modulates C3 deposition [26].
By means of cell surface staining for FACS analysis they are often defined as CD4+CD25+CD127lowCD3+ T-cells.
We then specifically addressed the effect of mutant huntingtin on internalization by examining levels of HER2 at the cell surface by flow cytometry analysis (Fig 6B).
