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Infected-U937 cells were selected for copGFP expression by cell sorting analysis.
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Such events can be detected by fluorescence-activated cell sorting analysis by virtue of their expression of GFP.
The phenotypic changes in DC subsets were measured by fluorescence-activated cell sorting analysis and the cytokine release was measured by multiplex assay or ELISA.
The frozen cell pellets of PBMCs were subsequently thawed and stained with an anti-phospho-ERK antibody (Cell signaling Technology, Inc, Danvers, MA, USA), followed by a fluorescein isothiocyaanate-conjugated secondary antibody detection by fluorescence-activated cell sorting analysis.
The cells were observed to be >90% CD11c positive by fluorescence-activated cell sorting analysis.
After negative selection, greater than 95% of the resulting cells were CD4+ positive by fluorescence-activated cell sorting analysis.
Cells were analyzed by fluorescence-activated cell sorting analysis using CellQuest software (BD Bioscience).
Viral titre was calculated by fluorescence activated cell sorting analysis of transduced HEK293T cells.
T-cell phenotypes and activation were assessed by fluorescence-activated cell sorting analysis.
One day later, monocytes subsets were assessed by fluorescence-activated cell sorting analysis.
Expression of Fas and TRAIL receptors (TRAILR1-4) was determined by fluorescence-activated cell sorting analysis.
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