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For experiments of cell culture of osteogenic differentiation, our results showed that DEX immobilization has more efficiency than the other group (such as both groups of CSF and CSF with free DEX, CSF/fDEX), which was demonstrated as indicated by cell image analysis and ALP activity assay.
This study was aimed at determining whether tumour DNA content measured by cell image analysis could provide additional prognostic information when compared to that provided by flow cytometry.
DNA ploidy measured by flow cytometry classified 46 tumours as diploid and 55 as aneuploid, whereas by cell image analysis 30 were diploid and 71 aneuploid (P less than 0.002).
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In vitro, we observed the effects of Chol-let-7a on HCC cells by living cell image analysis and transmission electron microscopy.
In this study, we have quantitatively estimated the time-sequential changes in MF contribution to cell plate development using live cell image analysis of tobacco BY-2 cell lines.
Cell Profiler cell image analysis software (developed by the Broad Institute) was used for the quantification of 53BP1 focus formation.
Results were quantified by measuring the percentage of the photographic field occupied by endothelial cells by image analysis.
This work was supported by F&M Hackman and Eyler funds, NSF-MCB-0448297, HHMI Undergraduate Science Program and the services provided by the Cell Imaging and Analysis Facilities and Services Core of the Environmental Health Sciences Center, Oregon State University, Grant Number P30 ES00210, National Institute of Environmental Health Sciences, National Institutes of Health.
During the blind methods comparison study, we found some events on the glass slide samples that meet the three CTC criteria but it was clearly not a full cell by brightfield image analysis.
High-magnification acquisitions were done in the cortical regions where Nkx2.1 was expressed and cell quantification by image analysis revealed that ketamine (100 μM) significantly increased the proportion of Nkx2.1+-cleaved caspase-3+ co-labeled cells (** P=0.0022, n=6; Figure 2j).
The uptake of TAT-SA was further examined in living HeLa cells by quantitative image analysis (n = 25 30) in the presence of different endocytic inhibitors.
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