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Another explanation is that the recovery kinetics after photobleaching may be influenced by cell cycle changes.
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These results indicated that glutamine promoted cell growth by inducing cell cycle changes.
Importantly, knockdown of USP15 had no significant effect on cell-cycle distribution in cells without DNA damage treatment, indicating that the effect of USP15 depletion on HR was not caused by cell-cycle change (Supplementary Fig. 1c).
We performed a controlled in vitro study on B16F10 melanoma cells to investigate cell viability and cell cycle changes by the Tripan Blue, MTT and cell quest histogram tests at 24, 48 and 72 h post irradiation.
Exogenous oleic acid markedly reversed the cell cycle changes produced by SCD1 inhibition demonstrating the critical importance of MUFA to cell cycle progression.
Subsequently, immunoblot analyses were performed to investigate whether the cell cycle changes mediated by genotoxic stress could be correlated to the expression of cell cycle regulatory proteins.
It's worth noting that the cell cycle changes caused by HCMV were related to alterations of cell cycle factors, including increasing of CyclinE transcription activity, activating cyclin-dependent kinases (Cdks) and reducing the abundance of Cdk inhibitors (CKIs) [ 11, 24, 25].
Since the cell cycle changes monitored by FCM in the receptor-positive breast carcinoma appeared prior to any reduction in the tumour size, the results suggest that FCM may prove a valuable method in the early detection of tumour response to hormone treatment in human breast cancer.
Cell cycle changes were detected by flow cytometric analysis.
Cell viability was assessed using a colorimetric XTT-based assay, apoptosis and cell cycle changes were monitored by FACS analysis.
Cell cycle changes were assessed by FACS analysis of PI-labelled cells and invasion and migration compared in quantitative Boyden chamber-based assays.
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