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PNP-GDEPT mediated enhancement of apoptosis was reinforced by cell cycle analyses.
We analysed the mode of cell death by cell cycle analyses employing propidium iodide.
Apoptosis was analyzed by cell cycle analyses, and apoptotic cells were clearly identified as cells with fragmented DNA (sub-G1 cells, Figure 1c, insets).
Expression of Nestin, NeuN, MAP2, and GFAP in ADSCs and terminally differentiated neurospheres was shown by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), western blotting, and immunocytochemistry; cell proliferation in neurospheres was evaluated by cell cycle analyses, immunostaining, and flow cytometry.
Comparable to wortmannin, MK-2206 resulted in a significant and dose-dependent enhancement of TRAIL-induced apoptosis in TRAIL-sensitive and in resistant melanoma cells, as determined at 24 h by cell cycle analyses (A-375, 48% Mel-HO, 24% A-375-TS, 28%).
These data are supported by cell cycle analyses (fluorescence-activated cell sorting, FACS) that show that at least 9% of S37P-hTERT cells continue to proliferate when in dense culture, while WT-hTERT cells enter the G0 phase (Supplementary Material, Fig. S7).
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Angiostatic potential of the RGD and Anx dual-conjugated liposomes was assessed by performing cell cycle analyses to investigate the antiproliferative effects induced by the synergistic effects of RGD and Anx.
Cell apoptosis was assessed by both cell cycle analyses of the sub-G1 population and caspase 3 cleavage assays.
CRITICAL STEP: Proper cultivation of pluripotent stem cells to keep cells healthy is essential because it can affect growth rates dramatically, which can be seen by some cell cycle analyses.
Normal cells (Fig. 1A and Fig. S1 [A]) and cancer cells (Fig. 1B and Fig. S1 [B]) were released from quiescence at G0/G1 phase and cell cycle profiles were determined by flow-cytometric cell cycle analyses.
The transfected cells were then incubated with H. pylori and the functions of HSP70 suppression were observed by viability assay, cell cycle analyses and TUNEL assay.
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