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For all centrifugation conditions studied in Figure 2, complete cell removal from the suspension was recorded by cell count analysis of the sample supernatant (data not shown).
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The cell number in each well was determined by parallel cell count analysis.
There was also no difference in 20 blood cell population (erythrocytes, leukocytes, and platelets) parameters, as assessed by complete blood cell count analysis (data not shown) [although there were some provocative differences due exclusively to the Ghr/bp gene disruption (Fig. S2)].
FoxP3+ nuclear presence per mm in tumor epithelium and surrounding stroma tissue was identified with the use of a Panoramic Midi scanner (3DHistech, Hungary) by means of an automated positive cell count analysis using AxioVision 4.6 (Carl Zeiss Vision, Jena, Germany).
15 µg of RNA was added per well (106 cells) and the cells were monitored by cell count, FACS analysis, western blotting and RT-PCR over a period of 7 days.
Cell counting analysis was used to confirm the MTT analysis.
Multinucleated clumps of cells were excluded from cell counting analysis.
A similar pattern was observed with platelet counts (Analysis 4.13), and white cell counts (Analysis 4.14).
Cell attachment was measured by cell count, DNA content analysis, and scanning microscopy.
By cell counting and FACS analysis we observed 4.8% and 3.9%, respectively, of CEBPα-positive cells, representing the combined adipocyte/preadipocyte population, to be positive for Ki67; we also found that 1.8% of CEBPα-positive cells incorporated BrdU per day (Ki67 labeling generally exceeds BrdU incorporation).
Mice were culled 1 and 2 days later, and the absolute number of cells of different phenotypes per pair of inguinal glands was enumerated by cell counting and flow-cytometry analysis.
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