Precursor cleavage was demonstrated by cell-surface biotinylation and Western blotting.
To determine whether this property is shared by ML352, we performed cell-surface biotinylation studies, testing ML352 after 15 min incubations, in parallel with vehicle, HC-3, or choline treated cells.
We have determined the distribution of NHE1 by means of immunofluorescence microscopy and cell-surface biotinylation.
Cell-surface biotinylation and detection of biotinylated CD6 was performed as described previously 33.
The biotin tracer assay was carried out using the modified cell-surface biotinylation method described by Umeda et al. (Umeda et al., 2006).
The cell-surface biotinylation method was adapted from a mammalian cell biotinylation assay.
As expected, the cell-surface biotinylation assay revealed that KL980 did not localize to cell surface.
Cell-surface biotinylation was done using a kit purchased from Pierce (#21335).
To quantify the amount of KL WT and KLΔ9 expressed on the cell surface, we conducted cell-surface biotinylation experiments.
A cell-surface biotinylation assay was used to determine the surface expression of GABAA receptor α1, α2, and α5 subunits.
The cell-surface biotinylation technique is more useful for quantification of a range of responses to modulators and inhibitors.
