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Our data argue that biallelic strict insulation at the ICR position is the cause of lethality in +/ mChβGI 2 pups by causing misexpression of at least one gene in addition to Igf2.
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This observation suggested that Cbx 1 was causing misexpression of Ubx.
It was, therefore, thought that the deletion caused misexpression of iab-5 in A4, perhaps by removing a repressor involved in iab-5 repression in segments anterior to A5.
The deficiency of Alkbh1 also causes misexpression of Bmp2, 4 and 7 at E11.5 during embryonic development.
Since either misexpression or loss-of-function of a gene can equally well address this question, a collection of P element insertion lines, most of which are expected to cause misexpression of genes, was chosen over more traditional approaches for several reasons.
Ablating maternal-zygotic expression of ZFP57 in mouse embryos causes misexpression of imprinted genes and loss of methylation at both paternally and maternally methylated ICRs.
To date, 17 different genes in Drosophila are classified as PcG members because mutations in these genes cause misexpression of HOX genes (reviewed in Schwartz and Pirrotta, 2007).
Our experiments demonstrate that PSR does not cause misexpression of annotated wasp chromatin-related genes, small RNA pathway genes, or TEs.
Mutations in many synMuv B genes also cause misexpression of germline-specific P granules in somatic cells and enhanced RNAi [ 37, 38].
Our results revealed that the mutation in the KG W chromosome caused misexpression of testis-specific genes, reduction of a set of piRNAs, and overexpression of transposons in the KG ovaries.
This lesion causes misexpression of neighbouring genes such that the expression of non-coding RNAs is elevated, and levels of delta-like homologue 1 (Dlk1), retrotransposon-like 1 (Rtl1) and deiodinase 3 (Dio3) transcripts are reduced.
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