Disruption of the target gene can occur by causing a shift in the reading frame, the generation of a premature termination codon, or the deletion of critical amino-acid residues.
There is also a 28 bp insertion near the end of exon 3 of A. syriaca (validated by Sanger sequencing) that causes a frame shift and the introduction of a stop codon.
In addition, we found another variant lacking the region encoded by exons 5 and 6, which causes a frame shift, resulting in a stop codon in the region encoded by exon 7. hNETΔEX6 mRNA was also detected in the adrenals and fetal brain but not in the adult brain, suggesting a tissue-specific and development-dependent splicing of hNET transcripts.
Empirically, a subjective quality degradation caused by a frame drop is more serious than that caused by spatial down-sampling at low bitrate.
As evidenced previously [ 4], incorrect annotation of the ORF is caused by a frame shift in the sequence presumably due to a missing base in the genome sequencing.
Further analysis of these sequences revealed that RXLR29 is represented by a null allele in Hpa Emoy2 caused by a frame-shift mutation resulting in a premature stop codon (Fig. S4).
DMD is most frequently caused by a frame-shift, nonsense, or duplication mutation in the DMD gene, which encodes dystrophin [ 27].
The inability of benzyl-α-GalNAc to affect CTB binding to Jurkat cells is consistent with the known O-linked glycosylation defect in these cells, caused by a frame-shift mutation in the gene encoding chaperone C1GALT1C1 (Ju and Cummings, 2002).
Make sure that the screw head won't stick out and interfere with operation of the door by causing binding or scarring the frame.
Patients with leptin deficiency, caused by a homozygous frame shift, nonsense, or missense mutation of the leptin gene, for example, have an undetectable level of leptin in the circulation and develop early-onset severe obesity.
