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The miR-RACE products were cloned and sequenced for confirmation where identical results were generated by both high throughput sequencing and miR-RACE, thus further confirming the power of Solexa technology in sequencing sRNA libraries for miRNA discovery as well as ascertaining the existence of these miRNAs.
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The results were confirmed by both high-throughput sequencing and RT-qPCR.
Detection of genome-wide SNPs by both high-throughput sequencer and typing array made it possible to evaluate genomic composition of genetically related rice varieties.
Twenty-two members in 4 miRNA families and 10 members belonging to 6 miRNA families were found to be up-regulated and down-regulated in response to drought stress by both high-throughput sequencing and RT-qPCR.
Moreover, it was predicted that modern breeding practices have generally decreased genetic diversity Detection of genome-wide SNPs by both high-throughput sequencer and typing array made it possible to evaluate genomic composition of genetically related rice varieties.
Here, we report the results of an optimisation of splicomer sequence design by the use of both high-throughput arrays and biological screens.
We validated the results from both high-throughput experiments by performing growth experiments in a BioScreen C instrument (Thermo Electron, Helsinki, Finland), which generates robust growth curves under more strictly controlled conditions.
Highly informative protein profiles for biomarker discovery have been generated by high throughput analysis of both gastric fluid and exfoliated cellular proteomes using small sample volumes and simple sample processing.
The sample was prepared by the high throughput printing process.
For BAC by BAC sequencing, tiling BACs into contiguous linear arrays is usually achieved by high throughput fingerprinting [6], [7].
Phenotypic screening of small molecule libraries by high throughput cell imaging.
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