Female Cx. pipiens were infected by intrathoracic inoculation for both determination of infectious dose-50 (Idose dose at which 50% of experimental hosts become infected) and growth of individual virus strains.
Instead, I was impressed by the determination of both cities to direct their profound legacies toward promoting peace for all mankind.
Examining a cohort of CKD and HD patients and a control group in a cross-sectional design, we assessed vitamin D status by separate determination of both 25-hydroxylated vitamin D metabolites (hydroxyergocalciferol, i.e., 25-OH D2, and hydroxycholecalciferol, i.e., 25-OH D3) in plasma.
This was corroborated by both TEM observations and by the determination of a very low dielectric percolation threshold (i.e., 0.3%).
Clubbing was assessed by clinical signs and by determination of the phalangeal depth ratio.
The antibacterial activities of both materials were measured by the determination of MICs and MBCs on Acinetobacter calcoaceticus, Citrobacter freundii, E. faecalis, Klebsiella pneumonia, P. acnes, Pseudomonas aeruginosa, S. epidermidis, and S. aureus.
Plant material precursors were characterised by analysis of molecular composition, by determination of bulk density and by scanning electron microscopy.
Calculation of the connexin distribution within the smooth muscle cell layer, the endothelial cell layer and between both layers was done by determination of the volume containing the positive staining of the smooth muscle cells followed by calculation of the content of Cx staining in this volume (software: Imaris, Bitplane).
Cellular protein was extracted and subjected to in vitro phosphorylation by specific PKC isoenzymes, followed by determination of telomerase activity.
The identity of both strains of each pair could be demonstrated by determination of phenotypic properties and by genotyping of the isolates.
