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Nodal support was represented by bootstrap replicates with the autoMRE convergence criterion (Pattengale et al. 2009).
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A phylogenetic tree using amino acid sequences of LKR/SDH from different sources by the neighbor-joining method verified the confidence of the branching order by 1,000 bootstrap replicates with the MEGA 4.0 software.
Brach supports (parsimony bootstrap: PB) were assessed by 1000 bootstrap replicates with 10 random taxon additions and heuristic search options.
Node support was assessed by 1,000 bootstrap replicates with the same model.
The sequences of silkworm SET-domain-containing proteins were applied to construct phylogenetic trees by neighbor-joining algorithm (1000 bootstrap replicates) with the program MEGA4.0.
Amino acid sequences of each homolog family were aligned with Muscle [ 48], and a neighbor-joining phylogenetic tree supported by 1000 bootstrap replicates was computed with FastTree [ 49].
Bootstrap support (BS) values for individual clades were calculated by running 1,000 bootstrap replicates of the data, with starting trees acquired by a single replicate of random stepwise addition of taxa under TBR branch swapping, with MulTrees ON.
Phylogenetic trees were generated by the neighbor-joining method using 1,000 bootstrap replicates by MEGA4 software with default parameters [ 26] and following the general approach previously used or comparison of shrimp retrotransponson-like elements [ 2].
To generate distributions on the set of clades inferred by a given strategy, we used the bootstrap, creating bootstrap replicates by randomly choosing with replacement B sets of L loci.
The E2 subset and NSP3 trees were generated by neighbor-joining analysis with 1,000 bootstrap replicates by using the Kimura 2-parameter model in MEGA2 (17 ).
The reliability of internal branches was assessed by bootstrapping, with 1000 bootstrap replicates, and the values are shown in percentages, with a branching cut-off at 50%.
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