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The robustness of the ML trees was statically evaluated by bootstrap analysis with 1000 bootstrap samples.
The reliability of the tree was assessed by bootstrap analysis with 1000 replications.
The tree topology of the neighbor-joining data was evaluated by Bootstrap analysis with 100 re-samplings.
The phylogenetic tree was constructed using MEGA 5.1 software (Mendez 2014), and the reliability of the tree topology was evaluated by bootstrap analysis with 1000 replicates.
The reliability of the dendrogram was tested by bootstrap analysis with 1.000 replicates using MEGA 6.0 (Felsenstein 1985 and Tamura et al. 2013).
Statistical support for the protein trees is given by bootstrap analysis with 100 resamplings.
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This observation was confirmed by bootstrap analysis done with pvclust [9] which allows the assessment of the uncertainty in hierarchical clusters (see Methods).
The entire genome region encoding the E2-K6-E1 proteinsequenceduenced or obtained from GenBank and a phylogenetic tree was constructed by the neighbor-joining and maximum parsimony methods followed by bootstrap analysis [37] with 1000 replicates to determine confidence values for the groupings.
Branch support was assessed by bootstrap analysis (1000 replica) with PAUP for MP, with PHYML (100 replica) [ 63] in Phylogeny.fr [ 64] for ML, and by posterior probability with MrBayes.
The robustness of the phylogenetic tree was evaluated by bootstrap analysis [ 55] with 1000 replicates using PAUP* 4.0b10.
Edge support was assessed by the bootstrap analysis with 1,000 replicates and by the approximate likelihood ratio test based on χ and Shimodaira–Hasegawa-like procedure.
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