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Robustness was assessed by bootstrap analysis using 100 replicates.
The confidence of each clade was estimated by bootstrap analysis using 1000 pseudo-replicates.
The confidence of the branches was evaluated by bootstrap analysis using 1,000 samplings.
Statistical support for the phylogenetic groups was assessed by bootstrap analysis using 1,000 replicate data sets.
13 The statistical robustness and reliability of the branching order within each phylogenetic tree was confirmed by bootstrap analysis using 1,000 replicates.
Sequence alignments were performed using ClustalW (27 ), and an unrooted phylogenetic reconstruction was performed using the neighbor-joining method followed by bootstrap analysis using the MegAlign V11 routine of the DNAStar program package (DNAStar, Madison, WI, USA).
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To classify SCLC from other lung cancer types, unsupervised clustering of the SSHN genes was performed by bootstrapping analysis using pvclust package [ 69].
The branch support for each tree was given by non-parametric bootstrap analysis using 1000 replicates.
All MELs were manually assessed using a genome browser and are shown in Supplementary file 3. The averaged fraction of overlapping regions for simulated MEL dataset were determined by 1000 cycles of bootstrap analysis using randomised equivalent number of fragments of identical sizes for each dataset distributed across the genome.
In order to verify the topology of the Bayesian phylogenetic trees supported by the posterior probability, we performed bootstrap analysis using the Maximum Likelihood method implemented in RAxML software [ 72].
Phylogenetic reconstruction was obtained by the neighbor-joining method [55] together with bootstrap analysis using 100 replicates.
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