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This observation was confirmed by bootstrap analysis done with pvclust [9] which allows the assessment of the uncertainty in hierarchical clusters (see Methods).
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Bootstrap analysis did not substantially alter the results (Table 1).
Bootstrap analysis did not support clustering of fungal or Dictyostelium sequences into distinct classes, but interestingly, ForC and ForG significantly clustered with the FHOD family (92% bootstraps).
The robustness of the ML trees was statically evaluated by bootstrap analysis with 1000 bootstrap samples.
Confidence was determined by bootstrap analysis (500 replicates)66 and trees displayed condensed at 70% confidence (percentage confidence values shown at each node) where stated.
The average repayment time by bootstrap analysis is 9.49 years and 9.45 by retrospective analysis.
Statistical support for each clade was assessed by bootstrap analysis (Felsenstein [1985]).
The reliability of the tree was assessed by bootstrap analysis with 1000 replications.
The numbers at the branches indicate the confidence level calculated by bootstrap analysis (1000).
Numbers at nodes (>50%) indicate support for the internal branches within the tree obtained by bootstrap analysis (percentages of 100 bootstraps).
The branches were supported by bootstrap analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com