Sentence examples for by boiling the samples from inspiring English sources
In the first step, a secondary layer of Mg(OH 2 was introduced by boiling the samples in NaOH solution.
Dry leaves (0.1 g) were extracted with 5 ml of 80% ethanol, by boiling the samples in glass tubes in a 95°C-water bath for 10 min.
The hydrolysis reaction was stopped by boiling the samples 5 min, followed by centrifugation at 10,000 rpm for 5 min.
Crude fiber was determined by boiling the samples in H2SO4 and KOH followed up by drying and ashing the samples at 500 °C for 2 h.
The reaction was terminated by boiling the samples in 1X Laemmli buffer with 0.2 M dithiothreitol (DTT) for 5 minutes.
Bound proteins were eluted by boiling the samples in SDS sample buffer and resolved by SDS/PAGE followed by immunoblotting with anti-EA antibody (1∶1000), anti-LEF-1 antibody (1∶1000) (BD Biosciences), anti-β-catenin antibody (1∶2000) (Santa Cruz Biotechnology), anti-α-catenin antibody (1∶2000) (GenWay).
The reaction was terminated by boiling the sample for 3 min, and the p-coumaric acid produced was measured by high-performance liquid chromatography (HPLC) (Waters, USA) comprising a Wufeng analytical instrument (Wufeng Co., Ltd ,China) and a symmetry reversed-phase C18 column (250 × 4.6 mm, 5 µm, pH 2 8, Waters, USA), according to Wang et al. (2013).
Unbound material was removed by washing five times with 1 ml lysis buffer J with 0.2% tween and protein was eluted by boiling the sample in one-sixth volume of 6X SDS-PAGE sample buffer.
After extensive washing with PBS buffer, PvNod41 that was bound to immobilized proteins on the matrix was recovered by boiling the sample with Laemmli buffer 2× [125 mM Tris-HCl pH 6.8, 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.02% (w/v) bromophenol blue] and analyzed by 12% SDS-PAGE and Coomassie Blue staining.
The reactions were stopped by immediately boiling the samples for 5 min. In protease-negative experiments, 30 µl of inactivated GI fluid was used (inactivated samples were prepared by heating chick intestinal tract fluid at 95°C for 15 min followed by cooling down on ice).
