Sentence examples for by boiling the sample from inspiring English sources

The reaction was terminated by boiling the sample for 3 min, and the p-coumaric acid produced was measured by high-performance liquid chromatography (HPLC) (Waters, USA) comprising a Wufeng analytical instrument (Wufeng Co., Ltd ,China) and a symmetry reversed-phase C18 column (250 × 4.6 mm, 5 µm, pH 2 8, Waters, USA), according to Wang et al. (2013).

Elution was performed by boiling the sample in SDS sample buffer.

Unbound material was removed by washing five times with 1 ml lysis buffer J with 0.2% tween and protein was eluted by boiling the sample in one-sixth volume of 6X SDS-PAGE sample buffer.

After extensive washing with PBS buffer, PvNod41 that was bound to immobilized proteins on the matrix was recovered by boiling the sample with Laemmli buffer 2× [125 mM Tris-HCl pH 6.8, 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.02% (w/v) bromophenol blue] and analyzed by 12% SDS-PAGE and Coomassie Blue staining.

The hydrolysis reaction was stopped by boiling the samples 5 min, followed by centrifugation at 10,000 rpm for 5 min.

In the first step, a secondary layer of Mg(OH 2 was introduced by boiling the samples in NaOH solution.

Crude fiber was determined by boiling the samples in H2SO4 and KOH followed up by drying and ashing the samples at 500 °C for 2 h.

Dry leaves (0.1 g) were extracted with 5 ml of 80% ethanol, by boiling the samples in glass tubes in a 95°C-water bath for 10 min.

The reaction was terminated by boiling the samples in 1X Laemmli buffer with 0.2 M dithiothreitol (DTT) for 5 minutes.

Bound proteins were eluted by boiling the samples in SDS sample buffer and resolved by SDS/PAGE followed by immunoblotting with anti-EA antibody (1∶1000), anti-LEF-1 antibody (1∶1000) (BD Biosciences), anti-β-catenin antibody (1∶2000) (Santa Cruz Biotechnology), anti-α-catenin antibody (1∶2000) (GenWay).

Proteolysis was quenched by boiling the samples for 5 minutes.