Sentence examples for by boiling in sample from inspiring English sources

The samples were eluted by boiling in sample buffer and protein levels were visualized via western blotting.

Finally, all fluid was removed and protein eluted by boiling in sample buffer.

Samples were prepared for SDS-PAGE by boiling in sample buffer.

After washing six times with lysis buffer, proteins bound to Sepharose beads were eluted by boiling in sample buffer, separated by 10% SDS-PAGE under non-reducing conditions, and transferred onto a nitrocellulose membrane (Trans-Blot transfer medium; Bio-Rad).

The reactions were precipitated with 40 µL protein G-agarose beads for 1 hour at 4°C and then washed five times with lysis buffer and eluted by boiling in sample buffer.

The isolated samples were frozen in liquid nitrogen, pulverized and lysed in lysis buffer, followed by boiling in sample buffer (50 mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.1% BPB, 2% 2-ME).

The complexes were released by boiling in sampling buffer without a detergent, separated by 7.5% SDS PAGE.

Crude protein extracts were prepared from 10 µl aliquots of the samples by boiling in SDS sample buffer (200 µl) for SDS-PAGE (62.5 mM Tris, 4% SDS, 25% glycerol, 0.002% bromophenol blue, and 5% β-mercaptoethanol, pH 6.8).

U2OS cells were harvested 48 h after transfection with expression constructs or miRNAs using Lipofectamine 2000, followed by protein sample preparation by boiling in Laemmli sample buffer.

After several washes with NETN buffer, samples were eluted by boiling in Laemmli sample buffer including β-ME and subjected to SDS-PAGE.

The reaction was terminated by boiling in SDS sample buffer after 5 15 min. Samples were separated by SDS PAGE, the gels were dried and analysed by autoradiography.