Sentence examples for by boiling each sample from inspiring English sources

The beads were washed twice with wash buffer (in mmoles: 25 Tris, pH 7.5; 30 MgCl2; 40 NaCl), and bound Rho was eluted by boiling each sample in Laemmli sample buffer.

Precipitated immunocomplexes were washed in the lysis buffer and either assayed for activity or subjected to immunoblot analysis by boiling samples in LDS sample buffer with reducing agent (Invitrogen).

Crude protein extracts were prepared from 10 µl aliquots of the samples by boiling in SDS sample buffer (200 µl) for SDS-PAGE (62.5 mM Tris, 4% SDS, 25% glycerol, 0.002% bromophenol blue, and 5% β-mercaptoethanol, pH 6.8).

Whole-cell lysates were prepared by boiling samples in SDS-PAGE sample buffer for 5 min, separated by SDS-PAGE, and transferred electrophoretically onto polyvinylidene difluoride membranes (Millipore Co).

U2OS cells were harvested 48 h after transfection with expression constructs or miRNAs using Lipofectamine 2000, followed by protein sample preparation by boiling in Laemmli sample buffer.

Samples were prepared for SDS-PAGE by boiling in sample buffer.

Finally, all fluid was removed and protein eluted by boiling in sample buffer.

After washing six times with lysis buffer, proteins bound to Sepharose beads were eluted by boiling in sample buffer, separated by 10% SDS-PAGE under non-reducing conditions, and transferred onto a nitrocellulose membrane (Trans-Blot transfer medium; Bio-Rad).

Elution was performed by boiling the sample in SDS sample buffer.

The antigen antibody complexes were isolated and dissociated by boiling in sample buffer for 4 5 min.

Bound proteins were eluted from beads by boiling in sample buffer (1 × Laemmli buffer).