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Exact(8)
The resulting polyclonal antibody (pAb) was specific as evidenced by blocking with the immunizing peptide and absence of reaction with C-terminal sucrase-isomaltase (Si) immunoprecipitates from the mouse mucosa.
The specificity of the affinity-purified antibodies was verified by blocking with the antigenic peptide using different methods (Fig. 2C, 4A) and by ELISA.
Anti-Tensin3 antibodies were tested for specificity using cell lysates expressing full-length recombinant Tensin3, as well as by blocking with the Tensin3 C-terminal peptide antigen (Figure S1).
Specificity of the NDRG1 antibody from Zymed® was confirmed by blocking with the peptide immunogen.
Specificity of the TTP antibody was determined by blocking with the identical peptide sequence used to generate the polyclonal antibody.
The specificity of the PTPRR antibody was confirmed by blocking with the immunising peptide (ag12145 Proteintech) (Additional file 1: Figure S2).
Similar(52)
These lanes were subjected to the same NHS/EDC activation followed by blocking with ethanolamine as the experimental lanes.
The specificity of recognition was confirmed by blocking with a CenH3 peptide, and the strength of the interaction was quantified by force measurements.
The primary antibody was a mouse anti-human mAb, followed by blocking with goat serum and the addition of a goat anti-mouse FITC-tagged secondary antibody.
Specificity was confirmed by blocking with NHS-acetate (Thermo), under the same conditions.
By blocking with one of your pieces.
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