Sentence examples for by blocking with from inspiring English sources

The slide was allowed to cool and washed twice with PBS followed by blocking with 3% normal human serum.

The non-specific binding, determined by blocking with excess cold-standard, was subtracted and only specific binding is shown.

The cells were then permeabilized in 0.1 % Triton X-100 in PBS for 5 min, followed by blocking with 1 % BSA for 20 min.

ZEGFR:2377-ST-[DyLight488] binding in A431 and FaDu cells was significantly reduced by blocking with excess ZEGFR:2377 (blue curves).

The cell uptake ranged from 10to15%5% per mg protein and could be reduced significantly by blocking with human minigastrin indicating specific receptor binding of all conjugates.

The resulting polyclonal antibody (pAb) was specific as evidenced by blocking with the immunizing peptide and absence of reaction with C-terminal sucrase-isomaltase (Si) immunoprecipitates from the mouse mucosa.

Heat-induced antigen retrieval was performed by microwaving in citrate buffer pH 6.0, followed by blocking with 10% normal goat or donkey serum, depending on the source of the secondary antibody, as described previously [31, 32].

Antibody specificity was tested by blocking with immunogen (see Figure S1 of Text S1).

MHC restriction of potential T-cell epitopes was assessed by blocking with anti-MHC or anti-CD4/anti-CD8 antibodies.

Non-adherent particles were removed by washing with PBS followed by blocking with 5% BSA in PBS for 1 hr.

The slides were then pre-treated with 0.5% peroxide followed by blocking with 10% normal goat serum.