Sentence examples for by blocking of non-specific from inspiring English sources

After deparaffinization, the antigen retrieval step was performed as listed in Table  1, followed by blocking of non-specific binding.

Grids were immunolabeled in a two step method according to the following procedure; the grids were conditioned in phosphate buffered saline (PBS) for 5 min ×3 at room temperature (RT), followed by the blocking of non-specific labeling for 30 min at RT using 5% non-fat dry milk in PBS.

Briefly, the sections were incubated in 0.05 M NaOH (pH 12.4) for 30 min at room temperature, followed by BSA-c as described above, for blocking of non-specific binding.

After blocking of non-specific binding sites by incubation with 10% FCS, the plates were washed with PBS and incubated with sera diluted in FCS.

Further blocking of non-specific antibody binding was then done by incubation with swine normal serum for 30 minutes.

After inhibition of endogenous peroxidase and blocking of non-specific reactions, primary antibodies were applied.

For membrane permeabilization and blocking of non-specific binding, preparations were permeabilized with 0.1% vol. Triton X-100 and 125 mM glycine (Carl Roth) in PBS for 30 min, followed by blocking with 3% BSA in PBS for 1 h.

50 μL HAB per tube was added again for blocking of non-specific binding.

After washing and blocking of non-specific binding, eotaxin dilutions in duplicate and samples were added.

Endogenous peroxidase activity was then blocked by incubation in 6% H2O2 in water for 10 min. Blocking of non-specific protein binding sites was performed by incubation in 10% normal goat serum for 10 min at room temperature.

Blocking of non-specific antibody binding was performed by incubation with normal goat serum at 37 °C.