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Exon inclusion can be achieved by blocking of intronic splicing silencer sequences as for the treatment of SMA.
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By blocking with one of your pieces.
This was followed by the "Coefficient of Variation" and then by ratio of intronic density to that of the intron with the minimal intronic density in the locus (Table 5).
We amplified by PCR all the exons of PAX8, TSHR, FOXE1, NKX2.1, NKX2.5 genes by means of intronic primers.
Alleles were distinguished by inspection of intronic sequence conservation, mapping to Assembly 2, and identity of neighboring genes.
Interestingly, induced hyperacetylation and in vitro reporter assays showed that NOS3 expression becomes self-attenuated by co-expression of intronic 27-nt-ncRNA modulating STAT3 signaling.
Variant 2 of CnAß has a unique C-terminal region generated by the translation of intronic sequences.
It suggested that a part of dysregulated genes might be mediated by aberrant DNA methylation of intronic miRNAs.
The location of the gene-trap vector was determined by PCR using combinations of intronic and vector primers.
Subsequent intronic -ORF loss resulted in pseudogenization of intronic -ORFs, followed by intron loss.
The retroviral RNA can be processed by cellular machinery and enables the removal of intronic sequences.
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