Sentence examples for by blocking of endogenous from inspiring English sources

Sections of 7 μm were subjected to fixation in acetone, blocking of endogen peroxidase in methanol and H2O2 followed by blocking of endogenous alkaline phosphatase by 20% acetic acid.

Frozen sections were fixed in acetone for 10 min followed by blocking of endogenous peroxidase with REAL™ Peroxidase-Blocking solution (DAKO Deutschland GmbH, Hamburg, Germany).

After being deparaffinized, sections were rehydrated in serial dilutions of ethanol and water; antigen unmasking was achieved using hot shock (for the antiphosphoNF-κB p65 antibody), followed by blocking of endogenous peroxidase using 3% hydrogen peroxide for 10 minutes.

Heat induced epitope retrieval using the microwave technique (citrate buffer pH 6.0, 20 min at 100°C) was followed by blocking of endogenous peroxidase with 3% H2O2 as well as endogenous biotin by an Avidin/Biotin-blocking Kit (Vector Laboratories, Burlingame, Ca, USA).

For E-cadherin immunohistochemistry, slides were blocked with 3%H2O22, followed by blocking of endogenous avidin and biotin.

Paraffin wax-embedded tissue sections were dewaxed with xylene, rehydrated through graded ethanols followed by blocking of endogenous peroxidase activity in H2O2/methanol for 12 min.

Briefly, the tissue sections were deparaffinized twice in xylene for 10 min each and rehydrated in a series of ethanol (100%-50%) and then subjected to antigen retrieval with 0.01 M citric buffer in a pressure cooker for 5 min, followed by blocking of tissue endogenous peroxidase activity with H2O2 treatment.

Sections were washed and endogenous peroxidase blocked in 1.5% H2O2/Tris-buffered saline (TBS) followed by washing and blocking of endogenous biotin using avidin-biotin (DakoCytomation, Glostrup, Denmark).

After dewaxing and blocking of endogenous peroxidases by incubation with 3%H2O22 for 30 minutes, the sections were incubated with nonimmune serum for 20 minutes.

After paraffin removal, hydration, heat-activated antigen retrieval in the DAKO-PT-link module (DAKO Glostrup, Denmark), and blocking of endogenous peroxidase activity by exposure to 3%% hydrogen peroxide for 20 min, the slides were incubated at 4 °C overnight with mouse anti-Cyclin A1 monoclonal antibody, clone 722407 (R&D Systems, Abingdon, UK) at a 1 25 dilution.

Sections were fixed in 4% PFA/TBS for 15 min, followed by incubation in 3% H2O2/TBS for 10 min to block endogenous peroxidase, and blocking of endogenous biotin (Avidin/Biotin Blocking System, DAKO).