Mechanistic studies suggested that the peptide acts by blocking a membrane fusion intermediate, although this was not unequivocally demonstrated, but defects were evident in late-stage entry steps.
Puckers involved in the transition between GlcNAc C1 and C4 chair conformers were largely precluded in GlcNS (except for SO), implying that N-sulfonation significantly slows the rate of C1 ↔ C4 conformational exchange by blocking these intermediates.
These data are consistent with Msh2 playing multiple roles in translocation formation by SSA; stabilizing dsDNA intermediates, facilitating the removal of non-homologous tails from dsDNA intermediates by Rad1-Rad10, and blocking an alternative mechanism for tail removal.
Interleukin-6- and leukemia inhibitory factor-induced terminal differentiation of myeloid leukemia cells is blocked at an intermediate stage by constitutive c-myc.
Likewise, PMS2 may modulate the efficacy of the repair machinery to process stalled replication forks arisen by blocked DNA-topoisomerase intermediates and gaps introduced by the chain-terminating antimetabolite gemcitabine.
Thus, we concluded that both compounds inhibit the sulfur transfer reaction from the persulfurated intermediate by blocking the binding of the sulfur acceptor to 3MST (Fig. 4c).
These compounds inhibit sumoylation by blocking the formation of SUMO-E1 thintermediatermedintermediate
The antibiotic inhibits the enzymatic activity of IARS by blocking the synthetic binding site of the intermediate Ile-AMP (Fig 3A) (Nakama et al, 2001).
Thus, CQ toxicity could be the result of either inhibiting autophagy in cells that require it for survival, or by blocking the autophagic process at a late stage, leading to intermediates that are toxic to the cells.
This compound was shown to inhibit HIV-1 maturation by blocking the protease-mediated cleavage of the CA SP1 processing intermediate to mature CA95,96.
