Sentence examples for by blasting their sequences from inspiring English sources

Primers were checked for their specificity by blasting their sequences against NCBI non-redundant sequence database.

In addition, probe sets showing trait dependent expression with bit scores below 50 were rechecked for their identity by blasting Affymetrix core sequences of these probe sets before functional annotation analysis.

Sequences were assembled using CLC Main Workbench 5.2 software (CLC bio, Aarhus, Denmark) and species identification was done by blasting the sequences in the NCBI database (http://www.ncbi.nlm.nih.gov/).nih.gov/

Specific PCR products from case samples were sequenced using the ABI BigDye3.1 and the breakpoints were determined by blasting the sequences with human reference genome assembly.

Primers were designed using Primer3 software (http://frodo.wi.mit.edu/primer3/) (Table 3) and primers specificity was evaluated by blasting primer sequences against the NCBI database.

Matching probeset IDs were obtained by blasting mRNA sequences against the Mt Affy Chip Consensus Sequences database on the M. truncatula Gene Expression Atlas website http://mtgea.noble.org/v2/.org/v2/

Primers for quantitative RT-PCR were designed using Primer5 software and primer specificity was evaluated by blasting primer sequences against the NCBI database.

By blasting the gSSR sequences to cotton ESTs, 367 gSSR sequences were matched to cotton ESTs.

Lung Cancer DSA research tool sequence content was annotated by blasting all target sequences against a series of public databases.

Consequently, plots for the frequency of 10 16 nt sequences were constructed for each species by blasting sequences with different proportions of the most common dinucleotides against the cDNA of each species (Fig.  2).

In addition, functional annotation was performed in Blast2Go (http://www.blast2go.com/) where gene information were obtain by blasting sequences in Gene Ontology (http://www.geneontology.org/).org/