Sentence examples for by biophysical methods and from inspiring English sources

The structural data are well confirmed by biophysical methods and mutant studies.

To better understand the structural consequences and rationalize the observed functional gains in these OYE1 variants, we selected representatives from sectors I III for further characterization by biophysical methods and X-ray crystallography.

The single-proline αSynuclein mutant A56P as well as the triple-proline mutant A30P/A56P/A76P (TP) showed drastically reduced propensity to form proteinase K (PK -resistant aggregates in vitro and in vivo, confirming the PK -resistanterizaggregateshe mutants as prefinrillar αSynuclein vitronts by biophysicandmethods and in invivoebrates [ 22].

Fascin-1 has been studied in cultured cells and by biophysical methods, but little is known about its distribution and functions in vertebrate development.

To investigate how the p.Trp43Arg mutation could lead to autosomal dominant congenital cataract, the recombinant WT and mutated γD-crystallin were overexpressed in E. coli, purified, and studied by biophysical methods.

Mainly in the 21st Century, these translocation processes were directly confirmed by biophysical methods such as nuclear magnetic resonance (NMR) and single-molecule techniques.

A series of trisubstituted naphthalimides have been synthesized and evaluated as telomeric G-quadruplex ligands by biophysical methods.

This shows the feasibility of further analysis of the supramolecular organization of SERT and probably other recombinant proteins in rhabdomere membranes by biophysical methods like cryo-electron microscopy [41] or atomic force microscopy [42], [43].

Individual exceptions exist, e.g. a large proportion of human and mouse PPIs in the BIND database identified by biophysical methods, where x-ray crystallography (MI:0114) alone represents around 30% of all PPIs; this reflects the inclusion in BIND of data from structural studies of protein complexes.

Thus, given the importance of expanded non-coding repeats in other RNA repeat expansion diseases15, we have investigated the secondary structure of the C9orf72 hexanucleotide repeat by biophysical methods to determine whether it forms RNA G-quadruplexes.

There was no evidence of KBU2046 binding to either CDC37 or HSP90β by biophysical methods, inclusive of isothermal titration calorimetry, fluorescence-based thermal shift assay, biolayer interferiometry, or by dynamic light scattering, nor by the biochemical method of drug affinity responsive target stability (DARTS) assay (Supplementary Fig. 14).