In the absence of editable substrates, ADAR2 is sequestered into the nucleolus by binding to nucleolar-enriched rRNA [ 47].
Erard, M. S., Belenguer, P., Caizergues-Ferrer, M., Pantaloni, A. & Amalric, F. A major nucleolar protein, nucleolin, induces chromatin decondensation by binding to histone H1.
These alterations unfold the NPM1c+ C-terminal domain with nucleolar displacement because of impaired nucleic acid binding.
Nucleolar-localized c-Myc is essential for ribosome biogenesis by binding to ribosomal DNA (rDNA), activating RNA polymerase I, and regulating the transcription of rDNA.
In fact, decreased CA IX binding to nucleolar chromatin observed in hypoxic cells was associated with decreased 45S rRNA precursor transcript levels.
These experiments suggest that Ki-67 directs the binding of nucleolar components to the chromosome periphery, perhaps by acting as a scaffold.
Nucleolin (NCL) is a nucleolar phosphoprotein involved in the synthesis and maturation of ribosomes, found associated with intranuclear chromatin and pre-ribosomal particles, which induces chromatin decondensation by binding to histone H1.
Note the probe's optical signal is brightest in the cell nucleus, with nucleolar labeling.
Effects of actD on nuclear structure were observed using immunofluorescence with nucleolar antibodies (Figure 4C) which showed typical disruption of nucleolar structure by drug treatment [46].
Our studies using single EGFP fusions demonstrated a strong nucleolar accumulation induced by the monopartite LANA NLS domains of KSHV (24RKRNRSP30) and RFHVMn (40ARKRRKG46) suggesting an underlying ability to interact with nucleolar components.
