We performed ChIP assay with ERα antibody followed by qPCR to confirm ERα binding to select binding sites of the above genes.
Using group data analysis (M-statistics), we detected increased binding to select arrayed proteins by IgM from the day 6 sera compared to the day -1 sera across all animals in the group, while IgG interactions with the orthopox proteins were negligible.
ERα binding to select genes that were alternatively spliced in response to E2 was verified by ChIP followed by real time PCR (ChIP-qPCR) as described previously [ 48].
Bovine S100A12 probably amplifies the inflammatory response via recruitment of neutrophils while bovine PTX3 activates the immune system by binding to C1q and selected pathogens.
Biotin-labeled phages were selected by binding to streptavidin.
In contrast, ItG parasites selected by binding to purified ICAM-1 protein displayed the 'bc' binding signature, which was not prevalent among parasites isolated from children with severe malaria (Figure 3).
For CML chronic phase samples, CD34+ cells from patients were selected by binding to immunomagnetic beads (MiniMACS; Miltenyi Biotec, Auburn, CA, USA) from resuscitation mononuclear cells, according to the manufacturer's instructions.
First, we selected clones using aerolysin, which kills cells by binding to their surface GPI-anchored proteins (20), to select clones that had defects in the transport of GPI-anchor proteins to the plasma membrane.
The hybridoma was selected by screening their secreted antibodies by binding to heterologous ES proteins (i.e., ES2, ES4), thus only selecting cells with 2F5 epitope-graft specificity.
If inside one of these windows we find a peak of our dataset we assume that the gene to which the transcript belongs is regulated by ERα binding to the selected peak.
