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Many of these microRNAs are thought to regulate the translational expression of other genes by binding to partially complementary sites in messenger RNAs.
These two proteins are members of the small heat-shock protein (sHsp) family and have the ability to operate as molecular chaperones by binding to partially unfolded target proteins and preventing them from aggregation [4] [7].
In animals, miRNAs usually repress the expression of target genes at the post-transcriptional level by binding to partially complementary sites in their 3' untranslated region (3'UTR).
2) The alternative and more common mechanism is by binding to partially complementary sequence in 3'UTR of target mRNA, leading to repression of protein translation [ 3, 8].
miRNAs are short non-coding RNAs that typically regulate gene expression at post-transcriptional level by binding to partially complementary sites of their target mRNAs.
As a part of this complex, the mature miRNA regulates gene expression by binding to partially complementary sequences in the 3′-untranslated regions (3′UTRs) of the target mRNAs, leading to mRNA degradation or translation inhibition [ 6].
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The fusion proteins C595scFv-Fc and C595scFv-Fc-IL2 C595scFv-Fc-IL2 C595scFv-Fc-IL2 ELISandor binding thepartially deglycosylated mAb1 antigen from human milk fat membranes as well as to a set of MUC595lycopeptides corresponding to parts of the repeat domain and containing the RPAP motif (Table 1).
34 Both nilotinib and dasatinib efficiently block Bcr-Abl tyrosine kinase catalytic activity by binding to distinct, partially overlapping sites in the kinase domain.
However, it was shown later that Munc18-1 also binds to the SNARE complex (Dulubova et al., 2007), and stimulates SNARE-mediated vesicle fusion in a reconstituted system, by binding to the partially assembled SNARE complex (Shen et al., 2007).
By binding to completely or partially complementary sites in the 3′-untranslated-region (3′UTR) of target mRNAs, miRNAs suppress the protein translation of these transcripts and/or degrade target mRNAs.
The cofactors that are kept within synapses by binding to SNAREs will be partially lost, which will result in a lower endocytotic capacity, and slower endocytotic kinetics.
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