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This pathway is commonly activated by binding of soluble ligands to death receptors (reviewed in [ 28]).
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The rennet whey content was determined in a neutralized TCA sample extract by binding of the soluble GMP to a peroxidase-labeled anti-bovine-κ-CN monoclonal antibody.
In contrast with other G protein-coupled receptors, PARs are not activated in vivo by binding of a soluble ligand but, instead, are activated by proteolysis triggered by extracellular proteases.
Some TNF receptors are strongly activated by the binding of soluble ligands, whereas others bind soluble ligands but then fail to trigger intracellular signaling.
ROS activation is triggered by receptor-mediated binding of soluble chemotactic agents, such as the bacterial peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) and receptor-independent phorbol ester phorbol-myristate-acetate (PMA).
Monoclonal antibodies against TNFα, such as infliximab (IFX) and adalimumab (ADA), block the biological functions of TNFα by binding to soluble TNFα and also transmembrane TNFα (mTNFα), 7 which induces complement-dependent cytotoxicity, antibody-dependent cell-mediated cytotoxicity 8 and outside-to-inside signalling.
Pretreatment of both gp120 IIIB and BaL by PJ inhibited subsequent binding of soluble labeled CD4 (Fig. 2).
Moreover, when the site is blocked by binding of another intrabody, VL12.3, turnover of soluble mHDx-1 in living cells is blocked.
However, it is not clear how closely measures of binding of soluble peptides to MHC class I molecules by in vitro affinity assays reflect peptide-MHC binding in DC.
The binding of soluble HIV-1HXBc2 gp120 to CXCR4-proteoliposomes and CD4-proteoliposomes was analyzed by FACS.
This data indicates that binding of soluble hsp60 does not alter ATP synthase activity.
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