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As a part of the Multinational Genome Sequencing Project of Brassica rapa, linkage group R9 and R3 were sequenced using a bacterial artificial chromosome (BAC) by BAC strategy.
The full genome sequence of inbred line B73 has been achieved using BAC by BAC sequencing strategy recently [ 23].
Furthermore, gene density was estimated as the number of genes on a BAC divided by BAC length.
For BAC by BAC sequencing, tiling BACs into contiguous linear arrays is usually achieved by high throughput fingerprinting [6], [7].
The BAC ends were reconfirmed by BAC end sequencing.
The BAC coordinates are deduced by aligning BAC fingerprints to the reference genome (Krzywinski et al., 2007) and are confirmed by BAC end sequencing experiments.
Similar(20)
The consortium responsible for the sequencing of the tomato (Solanum lycopersicum) genome initially focused on the sequencing of the euchromatic regions using a BAC-by-BAC strategy.
Sequence data have been assembled into contigs on a BAC-by-BAC basis and have immediately been released into the EMBL/Genbank/DDBJ DNA sequence databases.
The fully automated assembly and anchorage procedures were validated by BAC-by-BAC blast of the end sequences against the grape genome sequence, unveiling 7.3% of chimerical contigs.
The Maize B73 genome was sequenced in a bacterial artificial chromosome (BAC -by-BAC approach.
A high quality whole genome sequence of a japonica variety Nipponbare was determined by BAC-by-BAC sequencing [ 14], and a whole genome sequence of an indica variety 93-11 was determined by shotgun sequencing [ 15].
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