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By avoiding the solvent extraction of wood samples, enzymes could be screened on more commercially relevant feedstocks and enzymes resistant to wood extractives could also be discovered.
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It leads to improved safety of production by avoiding the use of low-boiling-point organic solvents such as petroleum ether and tert-butanol alcohol.
This can usually be prevented by avoiding lower volatile solvents.
Overall, testing itself must be sustainable and safe by avoiding solvents that may be hazardous or energy consuming, and testing should help to reduce the need of experimental animals.
A plate of 96 samples can be melted in 10 min, which is 100 times faster than DHPLC; mutation scanning by HRM avoids the use of solvents, maintenance of pumps and injectors, and the challenges with DNASep column.
This methodology offers significant improvements for the synthesis of pyrido[2,3-d]pyrimidine derivatives with regard to the yield of products, simplicity in operation, and green aspects by avoiding toxic catalysts and solvents.
This methodology offers significant improvements for the synthesis of bis indolyl)methanes with regard to the yield of products, simplicity in operation, and green aspects by avoiding toxic catalysts and solvents.
Gel drying assisted by supercritical fluids has been recently proposed to eliminate the organic solvents in a simple and effective step, avoiding the structure collapse.
The risk of cation loss may be diminished by avoiding evaporation of large quantities of high-boiling solvents.
The tubes were kept covered to avoid the loss of solvent due to evaporation.
Moreover, care was taken to limit Fischer esterification conditions during sample preparation by maintaining the pH of digestion buffers >8, by avoiding exposure of samples to high concentrations of methanol in the presence of acetic acid, and by avoiding methanol as a co-solvent during LC separation of the peptides.
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