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Bouton size analysis was performed by averaging two perpendicular diameter measurements of individual Ib boutons.
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All images displayed here were generated by averaging three consecutive images perpendicular to the viewing direction.
All images shown here were displayed by averaging three consecutive images perpendicular to the viewing direction.
In the RDS measurement, near-normal incidence reflectivity of two perpendicular directions was obtained in order to remove the influence of errors induced by optical components, averaging two spectra sample azimuth by 90°.
A positive result was defined as 3 mm or greater than negative saline control, determined by averaging maximal perpendicular wheal diameters 15 minutes after applying the lancet.
The average of two perpendicular diameters of the concrete spread after a full stop is considered as the slump flow value.
One observer positioned a 4.5 mm region of interest over the maximum pixel in the lesion and the average of two perpendicular diameters of the lesion was noted.
Tumor growth was monitored daily by measuring the average tumor diameter (two perpendicular axes of the tumor were measured by a caliper).
The manual image analysis was done by measuring two perpendicular diameters from the particle (Fig. 2) with DigitalMicrograph software (version 1.81.78) from Gatan Inc. and by evaluating the equivalent diameter of the particle size by the average of the measured dimensions.
Tumor growth was monitored by measuring two perpendicular diameters of the tumor using calipers.
Tumor size was monitored every two days by measuring two perpendicular diameters and the tumor volume was calculated using the formula: (width)2 × length.
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