Peripheral blood lymphocytes were isolated from Assaf breed ewes through a Ficoll-Paque density gradient; the purity of the cells (∼80%) was assessed by an automatic cell counter (Pentra 60, ABX, France).
A reliable direct method of measuring SCC is by using an automatic cell counter; either by using a portable cell counter at the farm, or by sending milk samples to a laboratory for measurement in, for example, a Fossomatic cell counter.
Cells were counted on days 3, 5, and 8 by using an Automatic Cell Counter (Millipore, Billerica, MA).
1. Complete Blood Count (CBC): Was measured by Sysmix K-21 automatic cell counter (Japan).
Cell counts were determined by a Cellometer® automatic cell counter (Nexcelom Bioscience, Lawrence, MA, USA) and 5 1000 cells were spiked into donor blood to estimate recovery yield.
Cell counts were determined by trypan blue staining followed by unbiased cell counting via EVE automatic cell counter (NanoEnTeK) at the indicated time points.
The numbers of viable and dead cells were automatically determined using an ADAM-MC automatic cell counter (NanoEnTek, Seoul, Republic of Korea) as previously described [ 17].
The cells that migrated into the lower chamber media were collected and counted by the Bio-Rad (Hercules, CA, USA) TC10 automatic cell counter.
Live cell proliferation assays were carried out by trypan blue dye exclusion using a Bio-Rad automatic cell counter (Bio-Rad, Berkeley, CA, USA).
The numbers of total (viable and dead) and dead cells were determined using an ADAM-MC automatic cell counter (NanoEnTek, Seoul, Republic of Korea) as described by Yi et al. [ 11].
