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Serum ALT and AST levels were measured by automatic biochemical analysis meter (Beckman, USA).
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LDH levels were evaluated by an automatic biochemical analysis system.
When tested by automatic biochemical analyzer, the frozen serum was restored to room temperature.
Serum creatinine, total cholesterol (TC) and triglyceride (TG) levels were measured by automatic biochemical analyzer (Hitachi 747, Tokyo, Japan).
Blood glucose, creatinine, total cholesterol, and triglyceride levels were measured by automatic biochemical analyzer (Hitachi 747; Hitachi, Tokyo, Japan).
Fasting blood total cholesterol, LDL-C, HDL-C, and triglyceride levels were measured by automatic biochemical analyzer (Hitachi 747; Hitachi Ltd., Tokyo, Japan).
Plasma Total Cholesterol (TC), TG, high density lipoprotein (HDL) and LDL levels were measured by automatic biochemical analyzer (HITACH17080, Japan) following the instructions of the company.
We measured the derivative of reactive oxygen molecules (d-ROMs), biological antioxidant potential (BAP) and superoxide dismutase (SOD) by automatic biochemical analyzer, malondialdehyde (MDA) and asymmetric dimethylarginine (ADMA) by enzyme-linked immunosorbent assay.
TET1 and TET3 re-localisation into the nucleus was further confirmed by the biochemical analysis of nucleus/cytoplasm fractions (Suppl. Figure 5d).
The molecular functions of Polδ had been revealed mainly by using biochemical analysis and yeast genetics.
We subsequently sought to validate the predictions of this modeling approach by further biochemical analysis.
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