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Quantification cycle (Cq) values [ 36] were determined by automated threshold analysis.
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For BVF analysis, the automated threshold function of MicroView was used for bone segmentation.
In Cohort I, the threshold as determined by RFC for the ER was associated with a similar HR for OS to that of the manual analysis and a 10% automated threshold.
For a discussion on the applicability of automatic thresholding see a study by Yang et al [ 37] in which several automated threshold selection methods are evaluated.
Epochs of analysis were visually inspected for oculomotor (saccades, and blinks), muscles, and other artifacts in addition to an automated threshold rejection criterion of 100 µV.
Briefly, it attempts to mimic Cumulus by using a fully automated thresholding procedure to perform the density readings.
We hypothesized that fixed "optimal thresholds" would be superior to commonly used "automated thresholds," which vary by each object examined, in a quantifying trabecular response to prostate cancer.
After deconvolution, automated thresholding was possible (F).
Vascular density was determined by automated image analysis.
After PCR, the absence of unwanted byproducts was confirmed by automated melting curve analysis.
The absence of unwanted by-products was confirmed by automated melting-curve analysis and agarose gel electrophoresis.
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