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A strong clue regarding the potential function of DUF2419 was provided by automated sequence profiling and structural modeling methods implemented in the program PHYRE2.
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Inserts from the positive clones were sequenced by automated sequence analysis.
The sequences of all mutants in the pEF-BOS vector were verified by automated sequence analysis.
Manual curation of Axnovel_3 revealed several small exons that were not identified by automated sequence alignments.
For the same reason, low-level heteroplasmy might be overlooked by automated sequence analysis.
The contigs for PGB02 (15 contigs) and PGB04 (14 contigs) obtained by automated sequence assembly were manually curated.
Homoplasmic 7-C sequence of BsaXI positive samples was confirmed by automated sequence analysis (Fig 1B, upper panel).
The orthologous groups in COG database were built by automated sequence searches, followed by careful manual curation for functional conservation.
The eluted fragment was sequenced by automated sequencing (Eurofins MWG).
All sequences were verified by automated sequencing.
All constructs were verified by automated sequencing.
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