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The contigs for PGB02 (15 contigs) and PGB04 (14 contigs) obtained by automated sequence assembly were manually curated.
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We found that most problems for automated sequence assembly resulted from chimeric clones in the plasmid libraries, bacterial DNA contamination, low-quality sequences and low-complexity repeats.
Most problems for automated sequence assembly resulted from chimerical clones in the plasmid libraries, bacterial DNA contamination, low-quality sequences, and low-complexity repeats.
These results illustrate some of the caveats to automated sequence assembly and annotation and highlight the necessity for corroboration after automated sequence processing when focusing on single genes or groups of genes of interest.
Inserts from the positive clones were sequenced by automated sequence analysis.
The sequences of all mutants in the pEF-BOS vector were verified by automated sequence analysis.
Manual curation of Axnovel_3 revealed several small exons that were not identified by automated sequence alignments.
For the same reason, low-level heteroplasmy might be overlooked by automated sequence analysis.
The eluted fragment was sequenced by automated sequencing (Eurofins MWG).
All sequences were verified by automated sequencing.
All constructs were verified by automated sequencing.
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