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Inserts from the positive clones were sequenced by automated sequence analysis.
The sequences of all mutants in the pEF-BOS vector were verified by automated sequence analysis.
For the same reason, low-level heteroplasmy might be overlooked by automated sequence analysis.
Homoplasmic 7-C sequence of BsaXI positive samples was confirmed by automated sequence analysis (Fig 1B, upper panel).
Actually, half of the mutations presented a level of heteroplasmy below 25%, which could be easily overlooked by automated sequence analysis.
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Analysis of the A TA nTAA motif in the promoter region of the UGT1A1 gene was performed by PCR, according to previous protocol (Monaghan et al, 1999), followed by automated sequencing analysis of the purified PCR products using an automated sequencer (ABI PRISM 3130XL DNA Sequencer; Applied Biosystems, Madrid, Spain).
After identification by automated sequencing analysis, the recombinant DNA plasmids transfected breast cancer cells using Lipofectamine™ 2000 Transfection Reagent (Invitrogen), according to the manufacturer's instructions.
25 The conformational epitopes on the protein surface are predicted by an automated sequence analysis of all phage display sequences and a comparison to the distributions of amino acids on three-dimensional patches on the protein surface.
Most recently, within the context of the European IMPACT project, PRINTS became more tightly integrated with InterPro: as well as providing new entries to the resource, a novel hierarchy-based filtering approach was developed, helping PRINTS meet InterPro's automated sequence analysis requirements by resolving protein family membership as unambiguously as possible.
Next-generation sequencing (NGS) has the benefit of high-throughput, automated sequence analysis, and single strand reads.
Manual curation of Axnovel_3 revealed several small exons that were not identified by automated sequence alignments.
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