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This was accomplished by automated injection of pharmaceutical modulators of mitochondrial oxidative phosphorylation fluxes to the medium; the specific compounds added, sequentially, were 1 μM oligomycin, 0.3 μM FCCP and 2 μM antimycin A. The chemical concentrations and PBMC seeding density were determined by titration.
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Briefly, 105 to 107 cells/25 µl per well of a 96 well plate were lysed in the same volume of cell lysis reagent by incubation for 5 min at RT. Fifty microlitres of the reconstituted luciferase reagent was added to the well by automated injection (BD Bioscience, Monolight luminometer).
The partnership aims to develop a deep understanding of the processes heralding an oncoming anaphylactic episode at the earliest stages in order to develop a wearable device for both rapid detection of anaphylaxis onset and automated injection of epinephrine.
Mass spectral analyses were carried out by three independent automated injections of 1 μl of each sample and were accepted only if the standard sample deviation was less than 1% of the normalized peak intensity.
Through the pigtail catheter, several automated injections of contrast media are performed to correctly evaluate the morphology of the aorta.
The accuracy of perfusion and permeability measurements is dependent on the compactness of the bolus injection of the contrast medium and in this regard, the recent availability of MR-compatible automated injection devices is welcome.
The only materials needed for this assay are donor and acceptor cells, a multi-plate reader capable of fluorometry and automated injection, C-solution, and I-solution.
Herein, we describe a microfluidic photoporation system for increased throughput and automated optical injection of cells.
The accuracy and reproducibility of binding is given by automated evaluation of optical density.
Renier, N. et al. Mapping of brain activity by automated volume analysis of immediate early genes.
In this system, a 2.7-Fr OCT imaging catheter is advanced distal to the lesion, and automated pullback is initiated in concordance with blood clearance by the injection of contrast media.
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