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These multichannel images were further quantified by automated image analysis algorithms, allowing concentration-effect curves to be generated and IC50 values to be calculated (Fig. 3B).
Härmä V, Schukov HP, Happonen A, Ahonen I, Virtanen J, Siitari H et al. Quantification of Dynamic Morphological Drug Responses in 3D Organotypic Cell Cultures by Automated Image Analysis.
Imaging the dynamics of tissue growth and morphogenesis is now possible with advances in time-lapse microscopy, but a true understanding of their complexities is limited by automated image analysis tools to extract quantitative data.
Here, we demonstrate the measurement of contact angles on wetted micron scale fibers by imaging the fluid meniscus with telecentric optics at a spatial resolution of 4 μm followed by automated image analysis.
Therefore, besides employing longitudinal, multi-animal imaging and efficient radiolabelling, overall study throughput was increased in our study by automated image analysis.
The transgenic zebrafish embryos were also considered to be experimental models compatible with EDA, and their integration in future studies is expected to be facilitated by automated image analysis procedures [52].
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In images obtained from immunohistochemistry, muscle fiber-type composition was also quantified by using automated image analysis (Leica QWin Standard v2.7; Leica Microsystems).
Foveal avascular zone (FAZ) area, FAZ circularity, total and parafoveal vessel density (VD), fractal dimension (FD), and vessel diameter index (VDI) from the superficial capillary plexus OCT-angiogram were measured by a customized automated image analysis program.
We have addressed this lack of high-throughput analysis tools by developing an automated image analysis tool optimized for use with trypanosomatid species.
Onboard automated image analysis by custom software incorporates functions for background smoothing, object finding, and enumerating fluorescent objects that grow in size and intensity over time.
Here, we combined confocal laser scanning microscopy with automated image analysis by a procedure that is knowledge-based, quantitative and adaptive to phagocytosis assays of any other A. fumigatus mutant.
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by computerized image analysis
by automated imaging analysis
by computer assisted image analysis
by computational image analysis
by quantitative image analysis
by automated image observation
by automated dialog analysis
by automated image image
by digital image analysis
by automated image field
by automated image cytometry
by specialized image analysis
by automated image collection
by video image analysis
by gel image analysis
by static image analysis
by automated sequencing analysis
by automated threshold analysis
by automated fragment analysis
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