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The anti-cancer properties of p53 were demonstrated by assessing the cell survival and growth rate which showed a positive linear correlation in cancer cells.
Cell cycle was analyzed by assessing the cell cycle phases using flow cytometry (FACScalibur, using Cell Quest software; Becton Dickinson) according to Nicoletti et al. [25].
Finally, the cellular immune responses induced by a single immunization with WSN/TatΔ51 59 virus were also evaluated by assessing the cell proliferation of spleen cells after in vitro stimulation with the Tat protein for 5 days.
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The authors verified this model as comparable to in vivo granulomas by assessing the aggregate cell types and cell differentiation through histology and immunostaining.
Afterwards, the anti-inflammatory activity was evaluated by grading the clinical signs and by assessing the inflammatory cell count, protein, PGE2 and TNFα levels in the aqueous humor.
Proliferation was determined by assessing the relative cell accumulation at different time points, as previously described.
This study was designed to establish the most suitable polyclonal stimulus in human peripheral blood mononuclear cells (PBMC) experiments by assessing the kinetics of cell viability, present immunophenotypes, proliferation, and cytokine production of the PBMC.
We further investigated this growth defect by assessing the position in the cell cycle of induced cells.
Complement-mediated cell lysis was measured by assessing the percentage of cells permeable to propidium iodide using flow cytometry, following exposure to rabbit serum (Serotec, Oxford, UK).
Following bone marrow transfer, we first confirmed the engraftment efficacy by assessing the proportions of cell populations that were positive for F4/80 and CD45.1 or CD45.2.
The number of dying cells was measured quantitatively by assessing the percentage of cells with fragmented, damaged or condensed nuclei.
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