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V-tip protein localization on the bacterial cell surface was measured by assessing the binding of a rabbit anti-PcrV IgG (1∶500) for 1 h.
We further investigated the effects of pH on NF-κB activity by assessing the binding of this transcription factor from nuclear extracts of mouse J774 macrophages to a consensus sequence oligonucleotide for the NF-κB promoter.
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The three biological replicate sets of Hmt1 binding site data generated were then coalesced by assessing the binding-site midpoint distances and merging peaks from at least two datasets that were less than 125 bp apart.
By comprehensively assessing the binding profiles of each heterodimer partner separately and then mapping their intersects, we were able to assess the functional importance of the heterodimer in PPARγ signaling and function.
DA release is measured with PET or SPECT by assessing the difference in binding potential for radiotracers that label DA D2/3Rs after a dopaminergic challenge as compared to a baseline condition [8],[21],[25].
It would seem straightforward to test this model by assessing the frequency of MT binding events or an assay similar to that used in Figure 1.
We tested whether their presence affects competitive binding by assessing the changes in U2AF65 occupancies in the HNRNPC knockdown.
We developed DrugFEATURE to quantify druggability by assessing the microenvironments in potential small-molecule binding sites.
A direct interaction was next explored by assessing binding to the ENTH domain of epsin 1 (with an His tag at the C-terminus) to increasing concentrations of the cytosolic portion of Syb2 fused to GST.
Therefore, we have examined further the DNA structure selectivity of BRCA1 230-534 by assessing its relative binding affinity for a range of DNA substrates recognised in DNA repair (figure 1).
Induction of apoptosis was assessed by the binding of annexin V-FITC/PI to phosphotidylserine.
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