Each patient was assessed by two randomly selected observers who independently scored the source of infection (by affected organ system or site), the plausibility of infection (rated as none, possible, probable, or definite), and the most likely causative pathogen.
The resulting double transgenic offspring were examined for CRISPR/Cas-induced somatic phenotypes, with germline mutagenesis assessed by mating randomly selected virgin females to e mutant males (w;;TM3/TM6b).
In the fourth Stage (reliability study), eight raters assessed thirty randomly selected decision support technologies.
Imputation accuracy was also assessed using randomly selected reference animals.
The quality control for these assays was assessed by randomly selecting 40 samples that were re-genotyped by an independent technician.
The accuracy of the interviews was assessed by randomly selecting 5% of the subjects to be re-interviewed 3 months after the initial interview.
Next, the expression levels of nearby genes (+/-100 kb) of the poised and active enhancer groups as defined above were assessed by randomly selecting 500 poised HSEL, 500 active HSEL, 500 poised MSEL, and 500 active MSEL.
Intraobserver repeatability of our areal BML measurement was assessed by randomly selecting 40 subjects with a BML to have their MRI scans re-read after at least a 2-week interval.
Loci were electrophoresed on an ABI Prism 3100- Avant genetic analyzer (Applied Biosystems Inc., Foster City, CA, USA), and alleles were scored using GENESCAN v3.7 software (Applied Biosystems Inc .. Genotype error rate was assessed by randomly selecting 100 individuals and performing a second DNA extraction and amplification of all twelve loci.
The consistency and validity of analysis and interpretation was assessed by having five randomly selected interview transcripts independently coded by two experienced qualitative researchers.
