Sentence examples for by assessing gene expression from inspiring English sources

By assessing gene expression through cDNA the strand information of the RNA is lost.

We tested this hypothesis by assessing gene expression of cathepsin K and RANKL in human disc tissue, and also applied immunohistochemistry analyses to disc tissues.

Having identified a set of core TGF-β/Smad3 target genes in the breast cancer model system using the ChIP-chip approach, we next determined which of these were specifically important for the tumor suppressive functions of TGF-β by assessing gene expression.

We then further ensured the on-target effect of these compounds by assessing gene-expression changes in MV4 11 cells.

Statistical veracity in assessing gene expression by qRT-PCR is an important component of the technique (Vandesompele et al. 2002; Silver et al. 2006).

A potential problem with assessing gene expression by mRNA quantitation is that this may not reflect cellular protein levels.

While informative, the small number of genes that can be assayed at one time makes assessing gene expression by qRT-PCR laborious and expensive, limiting is usefulness in phases of clinical research and medicine where a broader survey of gene expression is appropriate.

Using optimized formulations and transfection procedures, we assess gene expression by flow cytometry to specifically address some of the advantages and disadvantages of lipid-mediated RNA and DNA gene transfer.

We first assessed gene expression by RT-qPCR in 10 normal and 10 tumor derived pancreatic tissue samples as well as in 11 additional pancreatic cancer cell lines.

After 24, 48, and 72 hours, the cells were harvested for both mRNA and protein extraction to assess gene expression by quantitative real-time PCR (qRT-PCR) and immunoblotting, respectively.

This finding was rather expected, since the prognostic value of the markers examined remains contradictory, as supported in recent meta-analyses for ERCC1, which also stress the need for standardization methods to assess gene expression by qPCR in tumors [ 27, 30].