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The resulting sequence data was deconvoluted to individual BAC shotgun reads that then were assembled, first using the manufacturer supplied Newbler assembler and then by assembly with Phrap [ 41].
Poly ethylene glycol) (PEG) was incorporated into the multilayer architectures by assembly with biotin-derivatized poly l-lysine -g-poly ethylene glycol)(poly l-lysine -g-poly ethylene
Each oligonucleotide represented either a singleton (a single EST sequence) or a contig (contigous sequences of overlapping EST sequences) generated by assembly with CAP3.
These observations indicate that secondary germ cells develop from OSC in adult human ovaries and form new primordial follicles by assembly with granulosa cell nests.
The sequence data collected according to the Sanger protocol using a 3730xl capillary sequencer were subjected to trimming of sequences derived from cloning vectors with the Figaro and Lucy programs, followed by assembly with the PCAP.rep program.
High-quality reads of 14 carrot genotypes were then mapped to the genome sequences of 'DC-27' with TopHat (v2.0.10) and Bowtie2 (2.1.0), separately, followed by assembly with cufflinks (20, 21).
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Polyaniline (PANI) nanofibers are synthesized by dilute polymerization and subsequently used for layer-by-layer assembly with poly acrylic acid) (PAA).
The immobilization of these HPAs into water-processable films developed via layer-by layer assembly with polymer-stabilized silver nanoparticles led to the fabrication of the electrocatalytic interfaces for both nitrate and nitrite reduction.
The M. flocculare genome, which by assembly remained with 13 gaps, contains 585 coding sequences (CDSs), of which 356 have known functions and 229 are annotated as hypothetical.
We can then compare the expression estimates of transcript sets found by assembly methods with the estimates obtained using a simulated annotation for different levels of annotation accuracy.
The transcription start and termination sites were compared between genes identified by Tiling Assembly with those identified by the FL-cDNAs (Fig. 9).
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