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Here, we used RNA-seq for large-scale transcriptome profiling and generated a comprehensive transcriptome for W. somnifera by assembling the transcriptomes of in vitro adventitious root and leaf tissues from the millions of short sequence reads generated by Illumina.
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The aim of this study was to generate a resource for transcriptome profiling in P. patula by assembling the shoot transcriptome of this economically important species.
The initial transcriptome assembly was generated by assembling the Illumina reads using the CLC Workbench assembler.
AL assembled the transcriptomes.
DF assembled the transcriptomes and participated in writing the manuscript.
PASA was run twice to assemble the transcriptome data; first on EST data followed by UTR and isoform updates and then on the RNA-seq transcript assemblies.
SOAPdenovo was used to assemble the transcriptome sequences [ 40].
HG assembled the transcriptome and performed SNP discovery.
We aimed to address this by assembling a transcriptome for tuatara from an early-stage embryo, which will provide a resource for genome annotation, molecular marker development and studies of development and adaptation in tuatara.
We validated our pipeline by assembling three previous published transcriptomes and the transcriptome of the sepsid fly T. biloba, which was sequenced as part of this project.
Operons identified by assembling transcriptome data are indicated in yellow boxes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com